Conjugated surfactant micelles: A non‐denaturing purification platform for concentrated human immunoglobulin G

Production and purification of polyclonal antibody against F(ab')2 fragment of human immunoglobulin G

Antibodies are essential tools of biomedical and biochemical researches. Polyclonal antibodies are produced against different epitopes of antigens. Purified F(ab')2 can be used for animal’s immunization to produce polyclonal antibodies. Human immunoglobulin G (IgG) was purified by ion exchange chromatography method. In all stages verification method of the purified antibodies was sod...

Diffusion of Surfactant Micelles

Immunochemical properties of immunoglobulin G conjugated with lactate dehydrogenase.

Quantitative binding affinities of immunoglobulin G (IgG) for the five isoenzymes of lactate dehydrogenase (LD; EC 1.1.1.27) were determined for IgG isolated from three patients' sera that contained LD-IgG complexes. These three IgGs formed soluble complexes with four (LD 1, 2, 3, 5) of the five LD isoenzymes in two of the patients and in the third they bound with three (LD 2, 3, 4) of the five...

Protein A-conjugated luminescent gold nanodots as a label-free assay for immunoglobulin G in plasma.

We have employed protein A-modified gold nanodots (PA-Au NDs) as a luminescence sensor for the detection of human immunoglobulin G (hIgG) in homogeneous solutions. The luminescent PA-Au NDs were prepared simply by mixing protein A with the luminescent Au NDs (average diameter: ca. 1.8 nm). The specific interactions that occur between protein A and hIgG allowed us to use the PA-Au NDs to detect ...

Therapeutic surfactant-stripped frozen micelles

Injectable hydrophobic drugs are typically dissolved in surfactants and non-aqueous solvents which can induce negative side-effects. Alternatives like 'top-down' fine milling of excipient-free injectable drug suspensions are not yet clinically viable and 'bottom-up' self-assembled delivery systems usually substitute one solubilizing excipient for another, bringing new issues to consider. Here, ...